Polymorphism of waxy proteins in Spanish hulled wheats
نویسندگان
چکیده
Hulled wheats are neglected crops that have potential in plant breeding programmes of modern durum and common wheat. Among these wheats, three species were widely cultivated in Spain until the mid 20th century: Triticum monococcum ssp. monococcum (einkorn), Triticum turgidum ssp. dicoccum (emmer) and Triticum aestivum ssp. spelta (spelt). One important aspect of wheat grain quality is starch composition, which is related to the action of waxy proteins. A collection of 536 accessions of Spanish hulled wheats was analyzed for waxy protein composition using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Polymorphism was found for the Wx-A1, Wx-B1 and Wx-D1 proteins, including new and null alleles in the three species. An allelic variant with an electrophoretic mobility not previously described was found in einkorn wheat. In emmer and spelt, some alleles with different mobility were also found. A Wx-B1 null allele was detected in emmer wheat, and null alleles for Wx-A1, Wx-B1 and Wx-D1 were found in spelt wheat. The variations found could be used to enlarge the gene pool available to breeders, and to design new cultivars with different levels of amylose content.
منابع مشابه
Functional properties of waxy wheat flours: genotypic and environmental effects
A set of waxy (amylose-free) experimental spring wheats (Triticum aestivum L.) of diverse parentage were grown, along with two nonwaxy and two partial waxy check cultivars, at diverse North American cultural environments. Grain yield and functional attributes of derived flours were determined. Average grain yield of the waxy lines did not differ significantly from the average yield of the check...
متن کاملNitrogen Remobilization and N-P-K Concentration of Wheats of Different Ploidy Levels Respond Differently to Nitrogen Supply
Relationship of nitrogen (N) supply to nitrogen remobilization and leaf and grain P, K, and N status of wheat lacks clarity. The present pot experiment was conducted to evaluate nitrogen remobilization and leaf and grain P, K, and N status of ancient wheats of different ploidy levels in response to nitrogen. The experiment was carried out in Fall 2017-Spring 2018 at the Isfahan University of Te...
متن کاملmicroRNA172 plays a crucial role in wheat spike morphogenesis and grain threshability
Wheat domestication from wild species involved mutations in the Q gene. The q allele (wild wheats) is associated with elongated spikes and hulled grains, whereas the mutant Q allele (domesticated wheats) confers subcompact spikes and free-threshing grains. Previous studies showed that Q encodes an AP2-like transcription factor, but the causal polymorphism of the domestication traits remained un...
متن کاملFunctionality of chemically modified wild-type, partial waxy and waxy starchesfrom tetraploid wheats
Partial waxy (reduced-amylose) and fully waxy (amylose-free) tetraploid durum wheats (Triticum turgidum L. var durum) were developed by introgression of null alleles at the Wx-A1 and Wx-B1 loci from common hexaploid wheat (Triticum aestivum L.). Purified starches were obtained from each genotype, and chemically modified by: 1) cross-linking with phosphorus oxychloride, 2) substitution with prop...
متن کامل